Insertion junction: LMJ.RY0402.038901_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.038901
Chromosome:chromosome 7
Location:3867041
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g338900 (1 of 1) PTHR12072//PTHR12072:SF4 - CWF19, CELL CYCLE CONTROL PROTEIN // CWF19-LIKE PROTEIN 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACGGGCGCACAGCAGCTGGGCGGAGGAG
Internal bar code:TCTAGATTGGATCACCAGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:369
LEAP-Seq percent confirming:77.4704
LEAP-Seq n confirming:196
LEAP-Seq n nonconfirming:57
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTTCCCGGTCTTCTTCTC
Suggested primer 2:CAGCCCACAAACACATCAAC