| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038901 |
| Chromosome: | chromosome_7 |
| Location: | 3867041 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g338900 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCACGGGCGCACAGCAGCTGGGCGGAGGAG |
| Internal bar code: | TCTAGATTGGATCACCAGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 77.4704 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCCCGGTCTTCTTCTC |
| Suggested primer 2: | CAGCCCACAAACACATCAAC |