| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038902 |
| Chromosome: | chromosome_2 |
| Location: | 58971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g073500 | FAP241 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCCGCTGTGGACCTGCGCAACTCCGGCA |
| Internal bar code: | GGGACCGGCGTTGCCCGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1192 |
| LEAP-Seq percent confirming: | 96.1532 |
| LEAP-Seq n confirming: | 5699 |
| LEAP-Seq n nonconfirming: | 228 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCAGTTTGGTCACACAC |
| Suggested primer 2: | ATGTGCATGAGCTTGCTTTG |