| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038902 |
| Chromosome: | chromosome_2 |
| Location: | 573970 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g077600 | FAP51 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGCGCAACGACAGTGTATGTGTGTGCAT |
| Internal bar code: | ACACGTGGCGTACAGGGCTAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.3193 |
| LEAP-Seq n confirming: | 5850 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGGAGAACTGCATAGC |
| Suggested primer 2: | CTGGGTGCTGAGCATGAGTA |