| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038912 |
| Chromosome: | chromosome_3 |
| Location: | 4703432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g177750 | MAE10 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCTTGCACCTGTAGCCGCAAGCCGCAAG |
| Internal bar code: | CTGGCCCGCGCCGTTCCGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 99.0429 |
| LEAP-Seq n confirming: | 17592 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCCTCTTCACATGCTT |
| Suggested primer 2: | GGGGCATAGGTATTGGGTCT |