Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.038936 |
Chromosome: | chromosome_3 |
Location: | 3207528 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g165450 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGCCCTGATAGGGAGGAGGCGGGGGTTGG |
Internal bar code: | TTCGTGAACCTCGTGCGTCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 265 |
LEAP-Seq percent confirming: | 95.8138 |
LEAP-Seq n confirming: | 3067 |
LEAP-Seq n nonconfirming: | 134 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTTCAACTCCTCCACACCC |
Suggested primer 2: | GCTGCTAAAACCGTCAAAGC |