| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.038943 |
| Chromosome: | chromosome_1 |
| Location: | 1465344 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g007500 | NSS1 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCCGACTAGACCAAGACCCAGCCCGCCCA |
| Internal bar code: | GGTGGTACCATCGTGACGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 97.6959 |
| LEAP-Seq n confirming: | 1484 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAGATGAAGGTGGGACC |
| Suggested primer 2: | AGGTGTCCATCTTCGTCCTG |