Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.038945 |
Chromosome: | chromosome 17 |
Location: | 2129564 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAAATACAAAAGGGGCAAAGCAAGTCCG |
Internal bar code: | GTTATTAAACGCCTCCCCTGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1199 |
LEAP-Seq percent confirming: | 99.3111 |
LEAP-Seq n confirming: | 18308 |
LEAP-Seq n nonconfirming: | 127 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTAGGTGTGATGGACCC |
Suggested primer 2: | CCTCCCACTTTTAGCATGGA |