| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.038973 |
| Chromosome: | chromosome_4 |
| Location: | 2000080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g218800 | THB3 | Truncated hemoglobin | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGGTCTTCGTCGGAGCAGCACTACGCTG |
| Internal bar code: | ACCCTTAAAAGCTATGCCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 98.6709 |
| LEAP-Seq n confirming: | 1856 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTGATTGTATGTATGCG |
| Suggested primer 2: | CTACTCTGCTCACCGTTCCC |