Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.038978 |
Chromosome: | chromosome_3 |
Location: | 733521 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g146127 | FAP96 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTACATCACGGCGACAAGTACATTGACCCC |
Internal bar code: | AGTATGGATCGCCTGACTAAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 444 |
LEAP-Seq percent confirming: | 98.5255 |
LEAP-Seq n confirming: | 3742 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTCTGGGTTTACTTGGGT |
Suggested primer 2: | ACCCACAAGCTGTAACCTGG |