| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.038981 |
| Chromosome: | chromosome_9 |
| Location: | 3651583 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390467 | CGL66 | Predicted protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACATCGGGGGAGACAAACTCGCCGTAGCG |
| Internal bar code: | CGTATTGCGTCCCTCCTGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 634 |
| LEAP-Seq percent confirming: | 99.654 |
| LEAP-Seq n confirming: | 576 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGTCTGGGCCTAAACTT |
| Suggested primer 2: | ATTTCCATCCCAGTGAGTGC |