| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.039009 |
| Chromosome: | chromosome 7 |
| Location: | 5864984 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353650 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCGCTACCGCTCCCGCCGCCCGCCCT |
| Internal bar code: | AGGATCGCCCCGCCGGCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 92 |
| LEAP-Seq percent confirming: | 72.381 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTACCTTGGGGCAGTC |
| Suggested primer 2: | CTTCGTCCTAGGGGAGCAC |