Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.039041 |
Chromosome: | chromosome_6 |
Location: | 2938574 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g272800 | RPS8 | Ribosomal protein S8, component of cytosolic 80S ribosome and 40 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGGTTTCTCATTTACTCTCTAGACCATG |
Internal bar code: | GGGAGCCGCAAGGACATTGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 907 |
LEAP-Seq percent confirming: | 99.3408 |
LEAP-Seq n confirming: | 11152 |
LEAP-Seq n nonconfirming: | 74 |
LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGTTGACAAGAAAGGTGGT |
Suggested primer 2: | GGTAGGGGAATCAACGTGAA |