| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039046 |
| Chromosome: | chromosome 5 |
| Location: | 330572 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233303 | (1 of 1) K11718 - UDP-glucose:glycoprotein glucosyltransferase (HUGT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTGTTCCTGGATGTGCTGTTCCCGCTG |
| Internal bar code: | CCTGTTTGAGGGTAAGGCATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 98.2659 |
| LEAP-Seq n confirming: | 1020 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATACAACTCCGCCAGGT |
| Suggested primer 2: | GCGCTCATATCTCCTTTTGC |