| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039053 |
| Chromosome: | chromosome 14 |
| Location: | 466701 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATGACACGGTAGGTGTGAGCAACTGC |
| Internal bar code: | GATTCACTGAGCTGTGATCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1211 |
| LEAP-Seq percent confirming: | 95.9965 |
| LEAP-Seq n confirming: | 3261 |
| LEAP-Seq n nonconfirming: | 136 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACTTTCCAGAGGACCC |
| Suggested primer 2: | TATCTGCGTCGCGAGTATTG |