Insertion junction: LMJ.RY0402.039056_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.039056
Chromosome:chromosome_3
Location:3197264
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
None

Insertion site details

Flanking sequence (orientation from cassette outwards):GTTTCGTGCCCCGAGGGCGATGCCATGCTG
Internal bar code:TACGACGCCGCGGAACTCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:654
LEAP-Seq percent confirming:98.0807
LEAP-Seq n confirming:4548
LEAP-Seq n nonconfirming:89
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGCCCTTTCTAGGGTTTC
Suggested primer 2:GAGAGAGGAACGATGCTTGG