Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.039056 |
Chromosome: | chromosome_3 |
Location: | 3197264 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTCGTGCCCCGAGGGCGATGCCATGCTG |
Internal bar code: | TACGACGCCGCGGAACTCGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 654 |
LEAP-Seq percent confirming: | 98.0807 |
LEAP-Seq n confirming: | 4548 |
LEAP-Seq n nonconfirming: | 89 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGCCCTTTCTAGGGTTTC |
Suggested primer 2: | GAGAGAGGAACGATGCTTGG |