| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039061 |
| Chromosome: | chromosome_3 |
| Location: | 3819781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g170550 | POC4 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATCAAGAACATCCATATGGTCAGCGCCTG |
| Internal bar code: | GCTGGCCGCGAAGGCGCAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 90.6098 |
| LEAP-Seq n confirming: | 6166 |
| LEAP-Seq n nonconfirming: | 639 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTGGACGGATTTGAGA |
| Suggested primer 2: | GTGGTAAGCTGCTCCTCCAG |