| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039061 |
| Chromosome: | chromosome 3 |
| Location: | 3819781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170550 | POC4 | Proteome of centriole protein 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCGCCGTCTTCTTGATCAGCGCGCGG |
| Internal bar code: | ACCGAGATGCGGGTCAATTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.6425 |
| LEAP-Seq n confirming: | 5574 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTGGACGGATTTGAGA |
| Suggested primer 2: | GTGGTAAGCTGCTCCTCCAG |