| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039071 |
| Chromosome: | chromosome 12 |
| Location: | 4782631 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524150 | POLH2,POLI2 | (1 of 2) K03509 - DNA polymerase eta (POLH); DNA polymerase eta | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCACCGCTGGCCGCGCTGAGTGCGTC |
| Internal bar code: | CTCGAAACCGGCGGCCCTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.5249 |
| LEAP-Seq n confirming: | 6913 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTAAATACCAGCCCAA |
| Suggested primer 2: | ATTGGCGTGCATTTGATACA |