| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039072 |
| Chromosome: | chromosome 12 |
| Location: | 8161248 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551000 | (1 of 1) PTHR33966:SF1 - PROTEIN ODR-4 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTTGAGAGCGGGACTGCGGTGGGAAT |
| Internal bar code: | CACAGCGTAGACCGCCGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1055 |
| LEAP-Seq percent confirming: | 99.8396 |
| LEAP-Seq n confirming: | 6845 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCAAGAAACTCAAGCCG |
| Suggested primer 2: | GGTTAGTTGGCAGGTGTGGT |