| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039078 |
| Chromosome: | chromosome_2 |
| Location: | 710086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g078350 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGAAGGGACAGGCTCGCATTAGCGTTAC |
| Internal bar code: | CGTTAATCGGGTGATTTTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.6159 |
| LEAP-Seq n confirming: | 17117 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGAACGACACCAGCACC |
| Suggested primer 2: | CTGTGGCTTGAGGGCTACTC |