| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039093 |
| Chromosome: | chromosome_13 |
| Location: | 3687082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g588959 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACACATCCACGACGTTCAGCAGCTCAGCA |
| Internal bar code: | AAACTCGACCGGAAGGTCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.5462 |
| LEAP-Seq n confirming: | 3729 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCTGACAGGGCGGTTA |
| Suggested primer 2: | CTGCGATCCAAGTCAACTCA |