| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039097 |
| Chromosome: | chromosome_10 |
| Location: | 6132816 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g463800 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGCTTGTGTCTGCTGTGGACCTGCCTGCT |
| Internal bar code: | GGATCACGTAATATCCGTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 98.9251 |
| LEAP-Seq n confirming: | 9571 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGAAGTCCCTCACACCAT |
| Suggested primer 2: | CATGTACATAGTGGCCGTGG |