Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.039116 |
Chromosome: | chromosome_6 |
Location: | 2983776 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g273400 | TMC2 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCACACACATGCGTGTGTCTGCGTTTATAT |
Internal bar code: | GCGATCCCCGTGTCGGTAGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 80 |
LEAP-Seq percent confirming: | 99.2674 |
LEAP-Seq n confirming: | 271 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGATTGAGGTTGGCCATGTA |
Suggested primer 2: | CGTGTTGCTTGACCTACGAA |