| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.039123 |
| Chromosome: | chromosome_5 |
| Location: | 1591854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g243452 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGAGGAGTGGGCGGGCAACAAACCCATGA |
| Internal bar code: | GCGACGGTTACCTGTCTGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.5103 |
| LEAP-Seq n confirming: | 7722 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGGCTGTTCTCCCAAAC |
| Suggested primer 2: | CCAATGCTCTTGCTGCTACA |