| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039125 |
| Chromosome: | chromosome 12 |
| Location: | 5500771 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531000 | AMT1H,AMT8 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATGCAAGCCCGTCACGTCTCCGTGGCA |
| Internal bar code: | GACGCACGCGGGTCCCCCTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 77.5697 |
| LEAP-Seq n confirming: | 1947 |
| LEAP-Seq n nonconfirming: | 563 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCGGCTCAGATGCTTAT |
| Suggested primer 2: | GGAGGGGCCTATAGAACCTG |