| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039203 |
| Chromosome: | chromosome 8 |
| Location: | 4249767 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380763 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCATCATGGCTGGGGTGTTGCTCCTGCC |
| Internal bar code: | CCTCTCCGTCTTTCTCTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 94.2149 |
| LEAP-Seq n confirming: | 342 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTCCCTAACTTCGTTCG |
| Suggested primer 2: | GTCAGTAACGCGATCCCACT |