| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039215 |
| Chromosome: | chromosome 6 |
| Location: | 2778918 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271050 | URN1 | (1 of 1) 3.2.2.3 - Uridine nucleosidase / Uridine ribohydrolase; Inosine/uridine-preferring nucleoside hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGTCAAGGCTGGTTTAAGGCCGGAGCT |
| Internal bar code: | CTTCTGCGTTCACGGATCATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 98.9201 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCTCCACCCAGTATCAC |
| Suggested primer 2: | CAACTGATGTGTGGTGAGGG |