| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039259 |
| Chromosome: | chromosome 1 |
| Location: | 3585630 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023050 | UBX2 | (1 of 2) IPR006567//IPR018997 - PUG domain // PUB domain; Ubiquitin-associated protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGGATTGCGCCGTGGGTCTGTGCAGTG |
| Internal bar code: | GACTATTTGAGGCGGAGTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 95.6166 |
| LEAP-Seq n confirming: | 1636 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCAGGTGGTGGATATG |
| Suggested primer 2: | CAACGACACCCACATTTCTG |