| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039265 |
| Chromosome: | chromosome 6 |
| Location: | 6836553 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295150 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGTTTGTACCACCGGGCGAAGGCTGGG |
| Internal bar code: | GTTTGCCGCGAGGAGCTAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 664 |
| LEAP-Seq percent confirming: | 99.8684 |
| LEAP-Seq n confirming: | 759 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGGTGTAGCCCTCTTC |
| Suggested primer 2: | GTGTGTTTGTGTATTGCGGG |