| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039286 |
| Chromosome: | chromosome 9 |
| Location: | 7102423 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411751 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCACGCGTCCATTTCGCAGCCACCGGGA |
| Internal bar code: | TTAATGTCGTTCGTAAGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.2404 |
| LEAP-Seq n confirming: | 2221 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAAGGTTGCATGGGTTG |
| Suggested primer 2: | GTTGGGTTGGTTGCAAGTCT |