| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039294 |
| Chromosome: | chromosome 9 |
| Location: | 717556 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAGCTCCCGCTCACCGCTTGTGCAGG |
| Internal bar code: | AAGCCTCTGTGACGTCGTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 98.5663 |
| LEAP-Seq n confirming: | 1375 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACCTTGAAGCGGAGGGT |
| Suggested primer 2: | TGTATGCAAGGGCAACACAT |