| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039294 |
| Chromosome: | chromosome 9 |
| Location: | 744803 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402500 | ATPVA2,ATPvA2 | Vacuolar ATP synthase subunit A; (1 of 3) K02154 - V-type H+-transporting ATPase subunit a (ATPeV0A, ATP6N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGAGTGAGTCGTGCAAACCGTTGCGCGT |
| Internal bar code: | ACTTTCGCCCGGGCGTCGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 98.6437 |
| LEAP-Seq n confirming: | 6764 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAAGTTTAGGAGCAGGC |
| Suggested primer 2: | GGAAACCATCAAGAAAGGCA |