Insertion junction: LMJ.RY0402.039303_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.039303
Chromosome:chromosome 1
Location:6381039
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g045500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCCCCATCCCACAAGTATTATCGCTGG
Internal bar code:TTATCGGGCACGGGCGCTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:61
LEAP-Seq percent confirming:99.5413
LEAP-Seq n confirming:434
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCCACAAGAAAGGCTTG
Suggested primer 2:CCTCCCCGTTCCATTATTTT