| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039327 |
| Chromosome: | chromosome 5 |
| Location: | 2199924 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234652 | POB4,ZNG2 | Zn chaperone; (1 of 2) PTHR13748//PTHR13748:SF44 - COBW-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACAGGCGGGGGCGGCTCCGAAGTGTGT |
| Internal bar code: | TCACCCTATGACGGGGTTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 82.2222 |
| LEAP-Seq n confirming: | 222 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTCCTCCTACTCCCCAC |
| Suggested primer 2: | CACCAAGCTTCACTTCCCAT |