| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039333 |
| Chromosome: | chromosome 13 |
| Location: | 2409816 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579598 | SSS3B,SSS3 | (1 of 2) PTHR12526//PTHR12526:SF341 - GLYCOSYLTRANSFERASE // SUBFAMILY NOT NAMED; Soluble starch synthase III | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGACACGCGCGCGCGGTGTTTACCTGG |
| Internal bar code: | CTTTCAGTCGCGAACGGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 74.7068 |
| LEAP-Seq n confirming: | 1465 |
| LEAP-Seq n nonconfirming: | 496 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCATAATGTTTGCGTCTT |
| Suggested primer 2: | CACCCATATATCCATTCGGC |