| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.039362 |
| Chromosome: | chromosome 1 |
| Location: | 1486327 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007651 | PUS10 | (1 of 1) 5.4.99.27 - tRNA pseudouridine(13) synthase / tRNA Psi(13) synthase; RNA pseudouridine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGTTTGGAAGGAGCGCTTAGACGTT |
| Internal bar code: | CGTAGCGAATGGTCTGGCCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 93.6803 |
| LEAP-Seq n confirming: | 252 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACCTAATGTGCCTGTG |
| Suggested primer 2: | GCACACTCCTTCAGGAAAGC |