| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039367 |
| Chromosome: | chromosome 11 |
| Location: | 3239276 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479700 | DFO1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCCGCACAGTCCGTGCCCCAATGTGT |
| Internal bar code: | GCATCATAGGTAGTGATGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 91.1032 |
| LEAP-Seq n confirming: | 256 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAGGTAGCTCTCTGGGC |
| Suggested primer 2: | AGGTGTCTGTCCCAGTGTCC |