| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039409 |
| Chromosome: | chromosome 12 |
| Location: | 6394687 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537641 | LPT1,LAT1 | (1 of 1) K13519 - lysophospholipid acyltransferase (LPT1, ALE1); Lysolipid Acyltransferase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACACCGCGATGCACAACACAACCCAGT |
| Internal bar code: | CGCTTGCGAAGTCGAGCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1128 |
| LEAP-Seq percent confirming: | 99.7628 |
| LEAP-Seq n confirming: | 12198 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCTTCATCAAGCCCAA |
| Suggested primer 2: | ACAGCGCGTTATGAGGAGAT |