| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039414 |
| Chromosome: | chromosome 6 |
| Location: | 3041685 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274150 | HFO32,HFO6,HFO31,HFO43 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGACGAAAGCCAGGTGGAAGGGACACG |
| Internal bar code: | TTCCCTTTCCCCTGTTTCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 98.8713 |
| LEAP-Seq n confirming: | 438 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTGGGAAATGCAGGAG |
| Suggested primer 2: | ACCAACTCACATCGGTGACA |