| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039468 |
| Chromosome: | chromosome 16 |
| Location: | 970312 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648800 | (1 of 6) IPR000253//IPR008984 - Forkhead-associated (FHA) domain // SMAD/FHA domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCGTGCTCGGGCCAACCCGTCAGAGCC |
| Internal bar code: | CGCCTGGGCGCGGTCGATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGAATGGCTGAAGTCGC |
| Suggested primer 2: | CTGATGCCAAGGGTGATTTT |