| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039512 |
| Chromosome: | chromosome 9 |
| Location: | 5217347 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399402 | (1 of 2) PTHR24305//PTHR24305:SF65 - FAMILY NOT NAMED // CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCGGGGCCCCGCACCTGATTTTGCCAG |
| Internal bar code: | CAATACTGGGCTAGGTAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 99.7736 |
| LEAP-Seq n confirming: | 1322 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCTTCAAGCTCAGGTGC |
| Suggested primer 2: | TCCGGTTAATGCCCATACAT |