| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039522 |
| Chromosome: | chromosome 16 |
| Location: | 5301873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682450 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTGATAACGAGACCCTACCTGCTCTGC |
| Internal bar code: | GCTCGGCTAAGGGACTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 99.0813 |
| LEAP-Seq n confirming: | 24050 |
| LEAP-Seq n nonconfirming: | 223 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACAAAACTCGGAGAGCTG |
| Suggested primer 2: | CTGTGGATGCTCGAATTGTG |