| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039522 |
| Chromosome: | chromosome 16 |
| Location: | 5301877 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682450 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACTGAGGGTGCCAAGGGTTATCGTCCC |
| Internal bar code: | TCGAGTTGAAGTTCAGACTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.2268 |
| LEAP-Seq n confirming: | 1540 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGATGCTCGAATTGTG |
| Suggested primer 2: | GGACAAAACTCGGAGAGCTG |