| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.039530 |
| Chromosome: | chromosome 4 |
| Location: | 2527415 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222150 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCTATAGGCGTGGACGGGGAGCGTC |
| Internal bar code: | CGACTCGCGCTCAGGTGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 99.5764 |
| LEAP-Seq n confirming: | 5642 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGCTCACGTACTCCTC |
| Suggested primer 2: | GTCAGGGCAAGTAGAGGCAG |