| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039556 |
| Chromosome: | chromosome 1 |
| Location: | 6335845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045200 | (1 of 1) 5.3.99.6 - Allene-oxide cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGACAACGATTGTCTTCGCGCACAACT |
| Internal bar code: | GCACGATCTGCGTCCGATAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 99.7497 |
| LEAP-Seq n confirming: | 6776 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCTGCTCAAAATTGCT |
| Suggested primer 2: | CACGAGCGTTCCAGTAGTGA |