| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.039572 |
| Chromosome: | chromosome 16 |
| Location: | 1014925 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649100 | MAPKKK13 | (1 of 1) K04419 - mitogen-activated protein kinase kinase kinase 11 (MAP3K11, MLK3); Mitogen-Activated Protein Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGAGCTGGCGGAGGTGCGGCCCTCGC |
| Internal bar code: | GTTTCCGCTTGCGACTTGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 351 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGTACGGCAGGTCACT |
| Suggested primer 2: | CAAGGTGTGTGGTGAACTGG |