| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039597 |
| Chromosome: | chromosome 6 |
| Location: | 6804383 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295001 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACCTGTCGTGTCAAACCCTAGCGCCCA |
| Internal bar code: | ATGGAATGTGTAGGTACTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 98.318 |
| LEAP-Seq n confirming: | 2572 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGTTGGTTATGTTTTCA |
| Suggested primer 2: | GTGTTCAAGAGCACAAGGCA |