| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.039631 |
| Chromosome: | chromosome_14 |
| Location: | 2932133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g627576 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAGTGCGGGGAGGGGATGCAGGGAGAGGG |
| Internal bar code: | ATGGGTACTCGGATGAAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 83.8668 |
| LEAP-Seq n confirming: | 2594 |
| LEAP-Seq n nonconfirming: | 499 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | GGAACTGAAGCTTCTCCACG |