| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039634 |
| Chromosome: | chromosome 1 |
| Location: | 4583281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGAGCAGGGGCGGGAAAGTAACGGCA |
| Internal bar code: | TATATATTTTTCCGACTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 99.4661 |
| LEAP-Seq n confirming: | 4285 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGATCAAGCATGTCCTTC |
| Suggested primer 2: | CCGCTTCTGAACCTATCAGC |