| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.039689 |
| Chromosome: | chromosome_3 |
| Location: | 7875701 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g202550 | ELG11 | Exostosin-like glycosyltransferase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCTCCCACCCAACCCAACCCGCAGGTACG |
| Internal bar code: | TCCGGTTCCGGTTTGCTATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 97.9703 |
| LEAP-Seq n confirming: | 6661 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACACACACCATCAGTG |
| Suggested primer 2: | CTGCTTCTCAACATCACGGA |