Insertion junction: LMJ.RY0402.039689_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.039689
Chromosome:chromosome 3
Location:7875701
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g202550 ELG11 Exostosin-like glycosyltransferase 11; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCCCACCCAACCCAACCCGCAGGTACG
Internal bar code:TCCGGTTCCGGTTTGCTATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:870
LEAP-Seq percent confirming:97.9703
LEAP-Seq n confirming:6661
LEAP-Seq n nonconfirming:138
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACACACACCATCAGTG
Suggested primer 2:CTGCTTCTCAACATCACGGA